| 货号 | 34411S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky\Mink\S. cerevisiae |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE-Cy®7 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
| 参考文献 | 1 . Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9. 2 . Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50. 3 . Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704. 4 . Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5. 5 . Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8. |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE-Cy®7 Conjugate). |
