| 货号 | 52075S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79,86 |
| 参考文献 | 1 . Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2 . Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. 3 . Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. 4 . Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. 5 . Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6 . Darnell, J.E. et al. (1994) Science 264, 1415-21. 7 . Ihle, J.N. (1995) Nature 377, 591-4. 8 . Wen, Z. et al. (1995) Cell 82, 241-50. 9 . Yokogami, K. et al. (2000) Curr Biol 10, 47-50. 10 . Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Western blot analysis of extracts from untreated (-) or treated with IFN-α (100 ng/ml, 5 min; +) HeLa cells using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (HRP Conjugate). |
