Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate)
货号：
6894S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 6894S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved-PARP (Asp214) (D64E10) XP® Rabbit mAb #5625. |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 555 |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | 4C |
参考图片Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (right) using Cleaved-PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and α-Tubulin (DM1A) Mouse mAb #3873 (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，未处理（左）和Staurosporine #9953（右）处理，使用Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb兔单抗 (Alexa Fluor® 555 Conjugate)（红色）和 α-Tubulin (DM1A) Mouse mAb鼠单抗 #3873（绿色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
