Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Biotinylated)
货号：
9185S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 9185S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as unconjugated Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625. |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 89 |
参考图片Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated). Western blot 检测HeLa细胞提取物，使用Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Biotinylated)兔单抗分析未处理 (-)或Staurosporine #9953 (1 μM, 3 hr)处理的样品. |
