Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose® Bead Conjugate)
货号：
4070S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 4070S |
| 描述 | This Cell Signaling Technology (CST) antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The unconjugated Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 reacts with human, mouse, rat, hamster, Drosophila melanogaster, bovine and zebrafish phospho-Akt protein. CST expects that Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose Bead Conjugate) will also recognize phospho-Akt in these species. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/D.melanogaster/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose Bead Conjugate) immunoprecipitates endogenous levels of Akt only when phosphorylated at Ser473. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
参考图片Immunoprecipitation of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose Bead Conjugate) (lanes 3 and 4). XP® Rabbit IgG (DA1E) mAb Isotype Control (Sepharose Bead Conjugate) #3423 was used as a negative control (lanes 1 and 2). The western blot was probed using Phospho-Akt (Ser473) (587F11) Mouse mAb #4051. 免疫组化分析NIH-3T3细胞的提取物，分为未处理组和PDGF处理组，所用抗体为Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb兔单抗 (Sepharose Bead Conjugate) (泳道 3 和 4)。XP® Rabbit IgG (DA1E) mAb Isotype Control (Sepharose Bead Conjugate) #3423 作为阴性对照(泳道1 和 2). Western blot 采用Phospho-Akt (Ser473) (587F11) Mouse mAb鼠单抗 #4051。 |
