Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (Biotinylated)
货号：
8543S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 8543S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777.本CST公司生产的抗体在最佳条件下与生物素偶联。该生物素偶联的抗体具有和未偶联生物素的抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP® #3777相同的物种交叉反应特性。 |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Western blot analysis of extracts from A-431 cells, untreated (-) or treated with hEGF #8916 (100 ng/ml for 5 min) (+), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (Biotinylated).用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®（生物素化）对下列细胞提取物进行免疫印迹检测：经过或未经hEGF #8916 (100 ng/ml，5分钟) 处理的A431 细胞的裂解液。 |
