Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (Biotinylated)
货号：
5199S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 5199S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as unconjugated Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398. |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (Biotinylated) detects endogenous eIF2α protein only when phosphorylated at Ser51. This antibody does not recognize elF2α phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
参考图片Western blot analysis of extracts from NIH/3T3 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (Biotinylated). 使用Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb兔单抗 (生物素标记)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中Phospho-eIF2α (Ser51)蛋白水平，细胞分为未处理组和thapsigargin处理组。 |
