Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose®Bead Conjugate)
货号：
4091S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 4091S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation assays. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of Met protein only when phosphorylated at Tyr1234/1235. This antibody may cross-react with overexpressed tyrosine phosphorylated Src proteins. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
参考图片Immunoprecipitation of HCC827 cell lysates using Rabbit IgG (Sepharose Bead Conjugate) #3423 (lane 1) and Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose Bead Conjugate) (lane 2). The western blot was probed using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077.用Rabbit IgG (Sepharose Bead Conjugate) #3423 (第一道) 和Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose Bead Conjugate)（第二道）对HCC827细胞的裂解液进行免疫沉淀。蛋白质印记检测使用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077。 |
