Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate)
货号：
3985S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 3985S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescence of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858. |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 555 |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
参考图片Confocal immunofluorescent analysis of C2C12 cells, treated with either insulin (left) or LY294002, U0126 and Rapamycin (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (红色)和β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate) (绿色)，共聚焦免疫荧光观察Phospho-S6 Ribosomal蛋白在C2C12细胞中定位，分为胰岛素处理组（左图）和LY294002, U0126 and Rapamycin处理组（右图）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 |
