Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated)
货号：
4093S 基本售价：
4460.0 元 规格：
-
产品信息
概述| 货号 | 4093S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 reacts with human, mouse, rat and monkey phospho-Stat3 (Tyr705) protein. CST expects that Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated) will also recognize phospho-Stat3 (Tyr705) in these species. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
参考图片Western blot analysis of extracts from HeLa cells, untreated or IFN-α-treated, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for western detection.Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated)研究未经处理的和经IFN-α处理的 HeLa 细胞的细胞提取液。Streptavidin-HRP #3999 用于免疫印迹信号的检测。 |
