Mouse IL-3 Neutralizing (D6C1) Rabbit mAb
货号：
12222S 基本售价：
4382.0 元 规格：
-
产品信息
概述| 货号 | 12222S |
| 描述 | Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND50 in the range of 0.5-3.5 µg/ml. |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | N |
| 目标/特异性 | Mouse IL-3 Neutralizing (D6C1) Rabbit mAb binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF. |
| 使用方法 | N(1:1) |
性能| 供应商 | CST |
| 背景 | IL-3 is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support the culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3-specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2 and the PI3K/Akt pathway, and phosphorylation of Stat1/3/5/6 (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5). |
| 存放说明 | -20C |
参考图片The proliferation of BaF3 cells treated with increasing amounts of mIL-3 #8923 was determined. After a 72 hr treatment, cells were incubated with a tetrazolium salt and the OD450 was determined.经过浓度递增的mIL-3 #8923 处理后的BaF3细胞发生增殖。在处理后72h，细胞与一众四唑盐共孵育，并在OD450处检测。 |
The ability of Mouse IL-3 Neutralizing (D6C1) Rabbit mAb to inhibit mIL-3-induced BaF3 cell proliferation was assessed. Cells were incubated with increasing concentrations of antibody in the presence of mIL-3 #8923 (250 pg/ml). After 72 hr, viable cells were detected by incubation with a tetrazolium salt and the OD450 was determined.Mouse IL-3 Neutralizing (D6C1) Rabbit mAb具有抑制mIL-3诱导的BaF3细胞增殖的能力已被评估。细胞与浓度递增的抗体mIL-3 #8923 (250 pg/ml)共同孵育。72小时后，活细胞通过四唑盐的处理，在OD450进行检测。 |
