| 货号 | 64952S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | IHC-P/IF-IC |
| 使用方法 | IHC-P(1:200) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of COS-7 cells, mock-transfected (left), or transiently transfected with EGF Receptor vIII mutation construct (center), and mock-transfected A549 cells (right), using EGF Receptor vIII (D6T2Q) XP® Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. Note the lack of EGFR vIII staining in A549 cells expressing wild-type human EGFR. | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma using EGF Receptor vIII (D6T2Q) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma using EGF Receptor vIII (D6T2Q) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded EGF Receptor vIII transfected 293T cell pellets (left) and HCC827 (EGFR positive) xenograft (middle and right) using EGF Receptor vIII (D6T2Q) XP® Rabbit mAb (left and middle) and total EGF Receptor (D38B1) XP® Rabbit mAb #4267 (right). Note the lack of EGFR vIII staining in the HCC827 xenograft that is strongly positive for total EGFR. |
