| 货号 | 53348S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , IF-IC , ChIP |
| 目标/特异性 | Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb recognizes endogenous levels of histone H3 protein only when phosphorylated at Ser10. This antibody detects phosphorylation at Ser10 in the presence of acetylated or methylated Lys9, but not in the presence of phosphorylated Thr11. This antibody does not cross-react with histone H3 phosphorylated at Ser28. |
| 使用方法 | W IP IF-IC F ChIP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
| 参考文献 | 1 . Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2 . Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. 3 . Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5. 4 . Cheung, P. et al. (2000) Cell 103, 263-71. 5 . Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73. 6 . Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. 7 . Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13. 8 . Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 9 . Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 10 . Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85. 11 . Dai J et al. (2005) Genes Dev 19, 472–88 |
Confocal immunofluorescent analysis of mitotic HeLa cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from HeLa cells, either untreated or treated with Nocodazole #2190 (100 ng/ml, 16 hr), using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (upper) or Histone H3 (D1H2) Rabbit mAb #4499 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase. | |
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377, and Phospho-Histone H3 (Ser10) Antibody #9701. As shown, Phospho-Histone H3 (Ser10) (D7N8E) XP®Rabbit mAb detects histone H3 phosphorylated on Ser10, regardless of Lys9 acetylation and methylation, but does not detect phosphorylated Ser10 in the presence of phosphorylated Thr11. In addition, this antibody does not cross-react with histone H3 phosphorylated on Ser28. |
