| 货号 | 5335S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Western blot analysis of extracts from HeLa cells, untreated or TSA-treated (400 nM for 16 hours), using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (upper) and α-Tubulin (11H10) Rabbit mAb #2125 (lower). The acetyl-specificity of the antibody was verified by blocking with an acetyl- or non-acetylpeptide. 使用Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (上图)和α-Tubulin (11H10) Rabbit mAb #2125 (下图)，免疫印迹（Western Blot）分析HeLa细胞中Acetyl-α-Tubulin (Lys40)和α-Tubulin (11H10)蛋白水平。细胞分为untreated或TSA-treated (400 nM for 16 hours)。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A #9950 (right), using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)或treated with Trichostatin A #9950 (右图)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Trichostatin A (TSA) #9950 (1uM, 16 hours) using Acetyl-alpha-Tubulin (Lys40) (D20G3) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody. |
