| 货号 | 5625S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) F (1:800) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 89 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (green). Actin filament were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). | |
共聚焦免疫荧光分析HeLa细胞，未处理（左）和Staurosporine #9953（右）处理，使用抗体为Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb兔单抗（绿色），DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5 ®＃4084（荧光DNA染料）。 | |
