| 货号 | 3434S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from HeLa cells, treated with either 10 μM of MG132 (to accumulate hydroxylated HIF-1α) or 10 µM MG132 and 1 mM DMOG (to accumulate nonhyroxylated HIF-1α), using Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb (upper) or total HIF-1α Antibody #3716 (lower).使用Hydroxy-HIF-1α (Pro564) (D43B5) XP®兔单抗（上）或总HIF-1α 抗体 #3716（下）对经过10 μM MG132（累积hydroxylated HIF-1α)）或10 µM MG132 和1 mM DMOG （累积nonhyroxylated HIF-1α）处理的HeLa细胞进行western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells, treated with either 10 μM MG132 (left) or 10 μM MG132 and 1 mM DMOG (right), using Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red).使用Hydroxy-HIF-1α (Pro564) (D43B5) XP®兔单抗对经过10 μM MG132 （左）或10μM MG132 和1 mM DMOG （右）处理的HeLa细胞进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554鬼笔环肽（红色）标记。 |
