| 货号 | 5659S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Nonphospho-STEP (Ser221) (D74H3) XP® Rabbit mAb detects endogenous levels of STEP61 protein only when dephosphorylated at Ser221 and of STEP46 protein when dephosphorylated at Ser49. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:50) |
| 供应商 | CST |
| 背景 | Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of ERK activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer disease (1).Activity of STEP61 is reduced upon phosphorylation of Ser221 (Ser49 of STEP46) due to lower affinity for its substrates (2). |
| 存放说明 | -20C |
| 计算分子量 | 46, 61 |
Confocal immunofluorescent analysis of rat hippocampus (left) and striatum (right) using Nonphospho-STEP (Ser221) (D74H3) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对大鼠海马（左）和纹状体（右）使用Nonphospho-STEP (Ser221) (D74H3) XP?兔单克隆抗体（绿色）和α/β-Synuclein (Syn205)小鼠单克隆抗体#2644（红色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from rat brain and mouse brain using Nonphospho-STEP (Ser221) (D74H3) XP® Rabbit mAb. The nonphospho-specificity of the antibody was verified by preincubating the antibody without peptide, with STEP61 (Ser221) nonphosphopeptide, or with STEP61 (Ser221) phosphopeptide prior to incubation with the membrane.对大鼠和小鼠脑使用onphospho-STEP (Ser221) (D74H3) XP? Rabbit mAb进行Western blot分析。非磷酸化特异性由无肽段抗体预孵，及孵育前用STEP61 (Ser221)非磷酸化肽及STEP61 (Ser221)磷酸化肽预孵验证。 |
