| 货号 | 3777S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:1600) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Western blot analysis of extracts of BxPC-3 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower).用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®（上）或抗EGFR的抗体#2232 （下）对经过或未经EGF处理的BxPC-3细胞的提取物进行免疫印迹检测。 | |
Flow cytometric analysis of A549 cells, untreated (blue) or EGF-treated (green), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb compared to concentration matched XP® Rabbit (DA1E) mAb IgG Isotype Control #3900 (red). 用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®对经过（绿色）或未经（蓝色）EGF处理的A549细胞进行流式细胞术分析，并用浓度匹配的兔源同型对照抗体(DA1E)XP® #3900作为对照（红色）。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or EGF-treated (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®（绿色）对经过（右）或未经（左）EGF处理的HeLa 细胞进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色。 | |
Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, control (left) or λ phosphatase-treated (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb.用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®对石蜡包埋的HCC827细胞异种移植物进行免疫组化检测：对照（左），经λ磷酸酶处理（右）。 | |
Immunohistochemical analysis using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb on SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 (paraffin-embedded KYSE450 cell pellets, untreated (left) or EGF-treated (right)).用抗磷酸化EGFR (Tyr1068)的兔源单克隆抗体(D7A5) XP®对磷酸化EGFR免疫组化检测对照切片SignalSlide™#8102进行免疫组化检测：石蜡包埋的经过（右）或未经（左）EGF处理的KYSE450细胞团。 |
