| 货号 | 3398S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb detects endogenous eIF2α only when phosphorylated at Ser51. The antibody does not recognize elF2α phosphorylated at other sites. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or λ phosphatase-treated (right), using Phopsho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb. 使用Phopsho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb兔单抗，免疫组化分析人类结肠癌石蜡切片，细胞分为未处理组（左图）λ磷酸酶处理组（右图）。 | |
Western blot analysis of extracts from C2C12 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (upper) or eIF2α Antibody #9722 (lower). 使用Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (上图)和eIF2α Antibody #9722 (下图)，免疫印迹（Western Blot）分析在C2C12细胞系中Phospho-eIF2α (Ser51)和eIF2alpha蛋白水平，细胞分为未处理组和thapsigargin处理组。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb. 使用Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb. 使用Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb兔单抗，免疫组化分析人类淋巴瘤组织石蜡切片。 |
