| 货号 | 3077S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb detects endogenous levels of Met only when phosphorylated at Tyr1234/1235. This antibody may cross-react with overexpressed tyrosine phosphorylated Src proteins in Western blot. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:320) F (1:200) IF-IC (1:800) IHC-F (1:320) |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of cell extracts from HeLa cells, untreated or stimulated with HGF, using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (upper) and Met (25H2) Mouse mAb #3127 (lower).用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (上) 和 Met (25H2) Mouse mAb #3127 (下)对未处理的和HGF处理的Hela细胞的提取物进行免疫印迹检测。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb对石蜡包埋的人肺癌组织进行免疫组化检测：左，未经处理；右，λ phosphatase处理。 | |
Immunohistochemical analysis on Src-transfected NIH/3T3 cells, using a Phospho-Src Family (Tyr416) Antibody (left) or Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (right), indicating that the antibody does not cross-react with Src phosphorylated at Tyr416 via immunohistochemistry.用Phospho-Src Family (Tyr416) Antibody (左) 和Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb（右）对转染了Src的NIH/3T3细胞进行免疫组化检测，结果显示本抗体在免疫组化中不与Tyr416磷酸化的Src发生交叉反应。 | |
Immunohisochemical analysis of paraffin-embedded HCC827 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb对石蜡包埋的HCC827细胞异种移植物进行免疫组化检测。 | |
Immunohistochemical analysis of frozen MKN45 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb对MKN45异种移植物的冰冻组织进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded xenografts from 3T3-Met (left) and 3T3-Ron cells (right) using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb, indicating that this antibody does not cross-react with activated Ron by immunohistochemistry. Image courtesy of Pfizer, Inc. 用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb对石蜡包埋的3T3-Met (左) 和 3T3-Ron（右）细胞的异种移植物进行免疫组化检测，结果显示本抗体在免疫组化中不与激活的Ron发生交叉反应。图片来自Pfizer, Inc。 | |
Western blot analysis of purified active Ron kinase using a Phospho-Ron (Ser1349) Antibody (A), a Phospho-Ron (Tyr1238) Antibody (B), Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (C) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (D). This demonstrates that Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb does not cross-react with phospho-Ron by western analysis.用Phospho-Ron (Ser1349) Antibody (A), Phospho-Ron (Tyr1238) Antibody (B), Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (C) 和 Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (D)对纯化的激活态Ron激酶进行免疫印迹检测，结果显示免疫印迹检测中Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb不与 phospho-Ron发生交叉反应。 | |
Immunohistochemical analysis of paraffin-embedded papillary renal cell carcinoma using Phospho-Met (Tyr1234/1225) (D26) XP® Rabbit mAb.用Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb对石蜡包埋的人乳突状肾细胞癌组织进行免疫组化检测。 | |
Confocal immunofluorescence analysis of MKN45 cells, untreated (left) or treated with SU11274 (1 μM, 3 hours; right), using Phospho-MET (Tyr1234/Tyr1235) (D26) XP® Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测MKN45细胞：未处理的(左)和SU11274(1 μM, 3 hours) 处理的（右）细胞，使用抗体为：Phospho-MET (Tyr1234/Tyr1235) (D26) XP® Rabbit mAb。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Flow cytometric analysis of MKN-45 cells, untreated (green) or treated with SU11274 (blue).对MKN-45细胞进行流式细胞术检测：未处理的（绿色），SU11274 处理的（蓝色）。 | |
Immunohistochemical analysis using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb on SignalSlide™ Phospho-Met (1234/1235) IHC Controls #8118 [MKN45 cells, untreated (left) or SU11274-treated (right)]. |
