| 货号 | 5536S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 计算分子量 | 289 |
Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa 细胞， rapamycin处理 (#9904, 10 μM for 2 hours, 左),胰岛素处理(150 nM for 6 minutes, 中) 或胰岛素和 λ磷酸酶处理(右), 所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb (绿色)。肌动蛋白丝采用DY-554 phalloidin标记。蓝色伪彩是DRAQ5 ® #4084 (荧光 DNA 染料)。 | |
