| 货号 | 5482S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:500) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3. |
| 存放说明 | -20C |
| 计算分子量 | 46, 48 |
Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (left) or insulin (right), using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用LY294002#9901（左）或胰岛素（右）处理C2C12细胞，使用Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗（绿色）进行激光共聚焦荧光分析。用DY-554phalloidin（红色）标记肌蛋白丝。蓝色伪彩=DRAQ5® #4084 （荧光染料）。 | |
Western blot analysis of extracts from C2C12 cells, untreated or treated with LY294002 #9901, insulin, or both using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (upper) or total NDRG1 Antibody #5196 (lower). 未经LY294002 #9901, insulin或经两者共同处理后的C2C12细胞获得提取物进行western blot实验，使用Phospho-NDRG1 (Thr346) (D98G11) XP® 兔单抗(上) 或total NDRG1 抗体 #5196 (下). | |
Western blot analysis of extracts from A431 cells treated with hEGF #8916 (100 ng/ml, 30 minutes), with or without calf intestinal phosphatase and λ-phosphatase, using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (upper) or total NDRG1 Antibody #5196 (lower). 在牛小肠碱性磷酸酶和λ-磷酸酶存在或不存在的条件下使用hEGF#8916(100ng/ml，30分钟)处理A431细胞，获得提取物进行western blot实验，使用Phospho-NDRG1 (Thr346) (D98G11) XP® 兔单抗(上) 或total NDRG1 抗体#5196 (下)检测。 | |
Western blot analysis of HeLa cells treated with hEGF #8916 (100 ng/ml, 30 minutes), mock transfected or transfected with SignalSilence® NDRG1 siRNA I #6245 or SignalSilence® NDRG1 siRNA II #6257, using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). HeLa细胞经hEGF #8916 (100 ng/ml, 30 分钟)，未转染或转染SignalSilence® NDRG1 siRNA I #6245 或SignalSilence® NDRG1 siRNA II #6257后获得细胞提取物进行western blot实验，使用using Phospho-NDRG1 (Thr346) (D98G11) XP® 兔单抗 (上)或 β-Tubulin (9F3)兔单抗 #2128 (下)进行检测。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, wortmannin #9951 and U0126 #9903 (blue), using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb. 未处理（绿色）或经LY294002#9901，wortmannin#9951和U0126 #9903（蓝色）处理后的Jurkat细胞，使用Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗进行流式细胞分析。 | |
Immunohistochemical analysis of paraffin-emebedded human lung carcinoma using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb. 使用Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 [paraffin-embedded LNCaP cell pellets, control (left) or LY294002-treated (right)] using NDRG1 Antibody #5196 (top) or Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (bottom). SignalSlide® Phospho-Akt (Ser473) IHC对照#8101[石蜡包埋 LNCaP 细胞, 对照 (左) 或 LY294002-处理(右)] ，使用NDRG1 抗体#5196（上）或Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗（下）进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb. 对照（左）或λ 碱性磷酸酶处理（右）后，石蜡包埋的人宫颈癌组织，使用Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-emebedded mouse colon using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb. 使用Phospho-NDRG1 (Thr346) (D98G11) XP®兔单抗对石蜡包埋的小鼠结肠组织进行免疫组化分析。 |
