| 货号 | 4858S |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | WB(1:2000) IHC-P (1:400) F (1:25) IF-IC (1:100) IHC-F (1:400) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal protein (Ser235/Ser236) (D57.2.2E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-S6 Ribosomal protein (Ser235/Ser236) (D57.2.2E) XP® Rabbit mAb兔单抗（绿色），共聚焦免疫荧光观察磷酸化S6核糖体蛋白在HeLa细胞中定位，分为rapamycin处理组（左图）和20% 血清处理组（右图）。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析小鼠脾脏组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded A549 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析A549异种移植物石蜡切片，分为对照组（左图）和λ磷酸酶处理组（右图）。 | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析LNCaP细胞石蜡切片，分为对照组（左图）和rapamycin对照组（右图）。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析人类结肠癌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析人类乳腺癌组织石蜡切片，分别孵育对照多肽(左图)和Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (右图)。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb compared to a nonspecific negative control antibody (red). 与非特异阴性对照抗体 (红色)比较，使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002、Wortmannin和U0126处理组（蓝色）。 | |
Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中磷酸化S6核糖体蛋白(Ser235/236)和S6核糖体蛋白 (5G10)的水平，如图所示分别给予λ磷酸酶、20% FBS (20分钟)和100 ng/ml PDGF (20分钟)处理。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片。 |
