| 货号 | 5364S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D68F8) XP® Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244. |
| 使用方法 | WB(1:1000) IHC-P (1:2000) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Immunohistochemical analysis on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cell pellets -/+ LY294002) using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，免疫组化分析SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (石蜡包埋的LNCaP细胞沉淀物， -/+ LY294002处理)。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，免疫组化分析人类结肠癌组织石蜡切片，其中分别封闭孵育对照多肽(左图)和抗原特异的多肽 (右图)。 | |
Immunohistochemical analysis of paraffin-embedded LNCaP cell pellets, control (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，免疫组化分析LNCaP细胞沉淀的石蜡切片，分为对照组（左图）和rapamycin处理组（右图）。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded Rh30 xenograft, control (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，免疫组化分析Rh30异种移植物的石蜡切片，分为对照组（左图）和rapamycin处理组（右图）。 | |
Confocal immunofluorescent analysis of HeLa cells, insulin-treated (left) and LY294002-treated (#9901, right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗（绿色），共聚焦免疫荧光分析HeLa细胞系，分为胰岛素处理组(左图)和LY294002处理组(右图)。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from MCF7 and NIH/3T3 cells, treated with 100 nM insulin (10 min) or 20% FBS (30 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (下图)，免疫印迹（Western blot）分析MCF7和NIH/3T3细胞，如图所示分为100 nM insulin (10 min)处理组和20% FBS(30 min)处理组。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗，流式细胞术分析Jurkat细胞，细胞分为对照组(绿色)和LY294002、wortmannin和U0126处理组(蓝色)。 | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb. |
