| 货号 | 9145S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F/ChIP |
| 目标/特异性 | Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | WB(1:2000) IP (1:100) IHC-P (1:400) F (1:200) IF-IC (1:100) IHC-F (1:200) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Immunnohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织,图片显示了细胞核的定位。所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin embedded human breast carcinoma, specifically endothelial cells, untreated (left) or lambda phosphatase treated (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织尤其是内皮细胞，未经过处理(左图),经过lambda磷酸酶处理(右图), 所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。 | |
Western blot analysis of extracts from IFN-alpha treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb. Note that the basal phospho-Stat3 in A431 is detected by the antibody.Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb抗体研究IFN-alpha处理的Jurkat 细胞和 HeLa 细胞 (左图), EGF 处理 A431 细胞(右图)。注：A431 细胞中的本底phospho-Stat3 是用抗体检测。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.流式细胞仪研究未经处理的HeLa细胞(蓝色)和经IFN-α处理的HeLa细胞(绿色)。所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。 | |
Immunohistochemical analysis of frozen H1650 xenograft using Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb.免疫组织化学染色分析冷冻SKOV-3异种移植物,所用抗体为Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, IFN-alpha treated (left) or untreated (right), labeled with Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (green).共聚焦免疫荧光分析经IFNalpha处理的HeLa(左图)或者未经处理的 HeLa细胞(右图) ,Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (绿色)标记。 | |
Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine, using Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋Apc (min/+)小鼠肠。所用抗体为Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。Hep G2 细胞培养至4 x 106, 并经过夜饥饿或IL-6(100 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 或2 μl Normal Rabbit IgG #2729 抗体。用人源 IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 | |
Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right). |
