| 货号 | 41194S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | BCL6 (D4I2V) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total BCL6 protein. It is predicted to detect both human BCL6 isoform 1 and isoform 2. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkins lymphoma (1). TheBCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Ye, B.H. et al. (1993) Science 262, 747-50. 2 . Onizuka, T. et al. (1995) Blood 86, 28-37. 3 . Zollman, S. et al. (1994) Proc Natl Acad Sci U S A 91, 10717-21. 4 . Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75. 5 . Shvarts, A. et al. (2002) Genes Dev 16, 681-6. 6 . Moriyama, M. et al. (1997) Oncogene 14, 2465-74. 7 . Niu, H. et al. (1998) Genes Dev 12, 1953-61. 8 . Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13. |
Flow cytometric analysis of Ramos cells (green) and Jurkat cells (blue) using BCL6 (D4I2V) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). |
