| 货号 | 47084S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB |
| 目标/特异性 | GFAP (D1F4Q) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total GFAP protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 2 . Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93. 3 . Jessen, K.R. et al. (1990) Development 109, 91-103. |
Western blot analysis of extracts from mouse brain and rat brain using GFAP (D1F4Q) XP® Rabbit mAb (Biotinylated). |
