| 货号 | 77358S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Monkey |
| 应用 | F |
| 目标/特异性 | VISTA (D1L2G™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total VISTA protein. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7). |
| 存放说明 | 4C |
| 参考文献 | 1 . Wang, L. et al. (2011) J Exp Med 208, 577-92. 2 . Flies, D.B. et al. (2011) J Immunol 187, 1537-41. 3 . Lines, J.L. et al. (2014) Cancer Res 74, 1924-32. 4 . Wang, L. et al. (2014) Proc Natl Acad Sci U S A 111, 14846-51. 5 . Liu, J. et al. (2015) Proc Natl Acad Sci U S A 112, 6682-7. 6 . Le Mercier, I. et al. (2014) Cancer Res 74, 1933-44. 7 . Lines, J.L. et al. (2014) Cancer Immunol Res 2, 510-7. |
Flow cytometric analysis of human whole blood cells stained with VISTA (D1L2G™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) and co-stained with CD19-APC and CD14-APC. VISTA is present on CD14+ Monocytes (bottom right; upper right quadrant) and shows an increase in intensity when compared to the isotype control (bottom left; upper left quadrant). CD19+ B cells are negative for VISTA (top right; upper left quadrant) and do not show an increase in intensity when compared to the isotype control (top left; upper left quadrant). |
