| 货号 | 51374S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-F |
| 目标/特异性 | β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total β-amyloid peptide (Aβ). The antibody detects several isoforms of Aβ, such as Aβ-37, Aβ-38, Aβ-39, Aβ-40, and Aβ-42. This product detects transgenically expressed human APP in mouse models. |
| 使用方法 | IF-F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimers disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3 . Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4 . Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5 . Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6 . Iijima, K. et al. (2000) J Neurochem 75, 1085-91. |
Confocal immunofluorescent analsysis of brain sections from >12-month old wild-type (left) or Tf2576 (right) mice using β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 594 Conjugate) #8152 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
