| 货号 | 59238S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | All |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Cre Recombinase (D7L7L) XP® Rabbit mAb (PE Conjugate) recognizes transfected and transgenic levels of total Cre recombinase protein. |
| 使用方法 | F |
| 供应商 | CST |
| 灵敏度 | Recombinant |
| 背景 | Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitroandin vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Abremski, K. et al. (1983) Cell 32, 1301-11. 2 . Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309. 3 . Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14. 4 . Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10. 5 . Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6. 6 . Guo, F. et al. (1997) Nature 389, 40-6. |
Flow cytometric analysis of 293T cells, untransfected (left peak) and transfected with a construct expressing Cre recombinase (right peak), using Cre Recombinase (D7L7L) XP® Rabbit mAb (PE Conjugate). |
