| 货号 | 96866S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Ezh2 (D2C9) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total Ezh2 protein. |
| 使用方法 | F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50. 2 . Visser, H.P. et al. (2001) Br J Haematol 112, 950-8. 3 . Chen, H. et al. (1996) Genomics 38, 30-7. 4 . Tonini, T. et al. (2004) Oncogene 23, 4930-7. 5 . Müller, J. et al. (2002) Cell 111, 197-208. 6 . Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11. |
Flow cytometric analysis of human peripheral blood mononuclear cells untreated (left) and treated (right) with anti-human CD3 (10 μg/ml, coated plates) and anti-human CD28 (5 μg/ml, 3 days, 37ºC), using Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (top) or Ezh2 (D2C9) XP® Rabbit mAb (PE Conjugate) (bottom) and co-stained with an anti-human CD3 antibody. |
