AIF (D39D2) XP® Rabbit mAb (Sepharose® Bead Conjugate)
货号：
5939S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 5939S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of AIF. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AIF (D39D2) XP® Rabbit mAb #5318. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7). |
| 存放说明 | -20C |
| 计算分子量 | 67 |
参考图片Immunoprecipitation of Jurkat, NIH/3T3, and HeLa cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose Bead Conjugate) #3423 and AIF (D39D2) XP ® Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using AIF (D39D2) XP ® Rabbit mAb #5318 and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127. 免疫沉淀分析Jurkat、NIH/3T3和HeLa细胞提取物，使用Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423和AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate)兔单抗。免疫印迹使用AIF (D39D2) XP® Rabbit mAb兔单抗#5318和Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127。 |
