EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate)
货号：
8742S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 8742S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (D38B1) XP® Rabbit mAb #4267. |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 目标/特异性 | EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family. |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 594 |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | 4C |
参考图片Immunofluorescent analysis of A549 cells, untreated (left) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 15 min; +) (right), using EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).免疫荧光方法检测A549细胞，细胞未处理（左图）或用人表皮生长因子（hEGF）#8916 (100 ng/ml, 15 min; +)处理（右图），使用的抗体为EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) (红色).蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084。 |
