EGF Receptor (D38B1) XP® Rabbit mAb (Biotinylated)
货号：
6627S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 6627S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as unconjugated EGF Receptor (D38B1) XP® Rabbit mAb #4267.本CST公司生产的抗体在最佳条件下与生物素偶联。该生物素偶联的抗体具有和未偶联生物素的抗EGFR的兔源单克隆抗体(D38B1) XP®#4267相同的物种交叉反应特性。 |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | EGF Receptor (D38B1) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Western blot analysis of extracts from HeLa and A-431 cells using EGF Receptor (D38B1) XP® Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.用抗EGFR的兔源单克隆抗体(D38B1) XP®（生物素标记）以及亲和素偶联的辣根过氧化物酶#3999对HeLa 细胞和A431细胞的提取物进行免疫印迹检测。 |
