Jak2 (D2E12) XP® Rabbit mAb (Sepharose® Bead Conjugate)
货号：
4089S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 4089S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Jak2 proteins. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| 存放说明 | -20C |
| 计算分子量 | 125 |
参考图片Immunoprecipitation of extracts from K562 cells, using Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) (lane 2). XP® Rabbit IgG (DA1E) mAb Isotype Control (Sepharose Bead Conjugate) #3423 was used as a negative control (lane 1). The western blot was probed using Jak2 (D2E12) XP® Rabbit mAb #3230 and detected with Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 followed by Anti-mouse IgG, HRP-linked Antibody #7076.免疫共沉淀K562细胞的提取液，所用抗体为Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) (泳道2)。XP® Rabbit IgG (DA1E) mAb Isotype Control (Sepharose Bead Conjugate) #3423 作为阴性对照(泳道1)。western免疫印迹用Jak2 (D2E12) XP® Rabbit mAb #3230作为探针 并通过Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 及随后的Anti-mouse IgG, HRP-linked Antibody #7076来检测。 |
