LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate)
货号：
13173S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 13173S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 555 |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
| 存放说明 | 4C |
参考图片Confocal immunofluorescent analysis of HeLa cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (4 hr, middle), or untreated (right) using LC3A/B (D3U4C) XP®Rabbit mAb (Alexa Fluor® 555 Conjugate) #13173 (red) and β-Actin (8H10D10) Mouse mAb #3700 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
