Met (D1C2) XP® Rabbit mAb (Biotinylated)
货号：
8041S 基本售价：
4264.0 元 规格：
-
产品信息
概述| 货号 | 8041S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Met (D1C2) XP® Rabbit mAb #8198. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Met (D1C2) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total Met protein. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 140, 170 |
参考图片Western blot analysis of extracts from SK-BR-3 (Met-) and HT-29 (Met+) cells using Met (D1C2) XP® Rabbit mAb (Biotinylated).Western blot法检测SK-BR-3 (Met-)和 HT-29 (Met+)细胞的提取物，使用抗体为：Met (D1C2) XP® Rabbit mAb (Biotinylated)。 |
