| 货号 | 12430S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Smad1 (D59D7) XP® Rabbit mAb (Biotinylation) recognizes endogenous levels of total Smad1 protein. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad9 (Smad8) at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Hogan, B.L. (1996) Genes Dev 10, 1580-94. 2 . Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3 . Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92. 4 . Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7 . Alarcón, C. et al. (2009) Cell 139, 757-69. |
Western blot analysis of extracts from HT-1080 and C2C12 cells using Smad1 (D59D7) XP® Rabbit mAb (Biotinylated). |
