| 货号 | 4005S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated PTEN (D4.3) XP® Rabbit mAb #9188 reacts with human, mouse, rat and monkey PTEN protein. PTEN (D4.3) XP® Rabbit mAb (Biotinylated) also recognizes PTEN in these species. |
| 反应种属 | Human/Mouse/Rat/Hamster/MonkeyDog |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PTEN (D4.3) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total PTEN protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb (Biotinylated). western blot分析不同细胞系提取物，所用抗体为PTEN (D4.3) XP® Rabbit mAb (Biotinylated)兔单抗. | |
Immunoprecipitation of PTEN from HeLa cell lysates using PTEN (D4.3) XP® Rabbit mAb (Biotinylated) and Immobilized Streptavidin (Bead Conjugate) #3419, followed by western blot using PTEN (D4.3) XP® Rabbit mAb #9188 and Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677. 应用 PTEN (D4.3) XP® Rabbit mAb (Biotinylated)兔单抗 和固定化的Streptavidin (Bead Conjugate) #3419进行免疫沉淀分析HeLa细胞提取物中的PTEN ，然后再western blot 分析，使用的抗体为PTEN (D4.3) XP® Rabbit mAb兔单抗 #9188 和Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677。 |
