| 货号 | 97099S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | HEXIM1 (D5Y5K) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total HEXIM1 protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Ouchida, R. et al. (2003) Genes Cells 8, 95-107. 2 . Michels, A.A. et al. (2004) EMBO J 23, 2608-19. 3 . Yik, J.H. et al. (2003) Mol Cell 12, 971-82. 4 . Buratowski, S. (2009) Mol Cell 36, 541-6. 5 . Lenasi, T. and Barboric, M. RNA Biol 7, 145-50. 6 . Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42. 7 . Wada, T. et al. (1998) EMBO J 17, 7395-403. 8 . Yamada, T. et al. (2006) Mol Cell 21, 227-37. 9 . Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103. 10 . Ketchart, W. et al. (2011) Oncogene 30, 3563-9. 11 . Ogba, N. et al. (2008) Cancer Res 68, 7015-24. 12 . Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60. |
Flow cytometric analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (PE Conjugate) (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype Control (PE Conjugate) #5742 (red). | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with JQ1 (500nM, 72 hours; green), using HEXIM1 (D5Y5K) Rabbit mAb (PE Conjugate). |
