DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigmas Anti-FLAG®M2 Antibody) (Sepharose®Bead Conjugate)
货号：
70569S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 70569S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | All |
| 来源宿主 | Rabbit IgG |
| 应用 | IP |
| 目标/特异性 | DYKDDDDK Tag (D6W5B)Rabbit mAb (Binds to same epitope as Sigmas Anti-Flag® M2 Antibody) (Sepharose® Bead Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigmas Anti-FLAG® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
参考图片Immunoprecipitation of extracts from COS-7 cells, mock transfected (lanes 1, 3, and 5; -) or transfected with a construct expressing CBP/DYKDDDDK-tagged full-length human Arrb1 (lanes 2, 4, and 6; +) using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) #3423 (lanes 1 and 2) or DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) (Sepharose® Bead Conjugate) (lanes 3 and 4). Lanes 5 and 6 are 10% input. Western blot analysis was performed using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) (HRP Conjugate) #86861. |
