| 货号 | 25809S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2 . Durbin, J.E. et al. (1996) Cell 84, 443-50. 3 . Meraz, M.A. et al. (1996) Cell 84, 431-42. 4 . Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7. 5 . Frank, D.A. (1999) Mol. Med. 5, 432-456. 6 . Wen, Z. et al. (1995) Cell 82, 241-50. |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with hIFN-α1 #8927 (green), using Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (PE Conjugate). |
