| 货号 | 17507S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | M |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-IC |
| 目标/特异性 | ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total ASC/TMS1 protein. |
| 使用方法 | IF-IC(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Masumoto, J. et al. (1999) J Biol Chem 274, 33835-8. 2 . Conway, K.E. et al. (2000) Cancer Res 60, 6236-42. 3 . Terasawa, K. et al. (2004) Clin Cancer Res 10, 2000-6. 4 . Stone, A.R. et al. (2004) Am J Pathol 165, 1151-61. 5 . Guan, X. et al. (2003) Int J Cancer 107, 202-8. 6 . Moriai, R. et al. (2002) Anticancer Res 22, 4163-8. 7 . Virmani, A. et al. (2003) Int J Cancer 106, 198-204. 8 . Das, P.M. et al. (2006) Mol Cancer 5, 28. 9 . Strong, R. et al. (1991) Brain Res 542, 23-8. 10 . Ohtsuka, T. et al. (2004) Nat Cell Biol 6, 121-8. 11 . Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22. |
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) differentiated with mM-CSF (20 ng/mL) for 8 days, then, either treated with LPS (50 ng/mL, overnight, left), or LPS (50 ng/mL, overnight) followed by Nigericin (10 uM, 2 hours, right), using ASC (D2W8U) Rabbit mAb (Mouse Specific) (Alexa 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and Nigericin (white arrows). |
