| 货号 | 48006S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | M |
| 来源宿主 | Rabbit IgG |
| 应用 | F CS-CA |
| 目标/特异性 | RIP3 (D4G2A) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total RIP3 protein from mouse. |
| 使用方法 | F(1:50) CS-CA (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. 2 . Hsu, H. et al. (1996) Immunity 4, 387-96. 3 . Stanger, B.Z. et al. (1995) Cell 81, 513-23. 4 . Ting, A.T. et al. (1996) EMBO J 15, 6189-96. 5 . Kelliher, M.A. et al. (1998) Immunity 8, 297-303. 6 . Devin, A. et al. (2000) Immunity 12, 419-29. 7 . Zhang, S.Q. et al. (2000) Immunity 12, 301-11. 8 . Lin, Y. et al. (1999) Genes Dev 13, 2514-26. |
Flow cytometric analysis of Neuro-2a (blue) and L-929 (green) cells using RIP3 (D4G2A) Rabbit mAb (PE Conjugate). | |
CellSimple™ cell-based analysis of Neuro-2a (blue) and L-929 (grey) cells using RIP3 (D4G2A) Rabbit mAb (PE Conjugate). |
