| 货号 | 37020S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | TRAIL (C92B9) Rabbit mAb (PE Conjugate) detects endogenous levels of total human TRAIL protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (10-13) and DcR2 (TRAIL-R4, TRUNDD) (14-15) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (16). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (17-18). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Wiley, S.R. et al. (1995) Immunity 3, 673-82. 2 . Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90. 3 . Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48. 4 . Pan, G. et al. (1997) Science 276, 111-3. 5 . Walczak, H. et al. (1997) EMBO J 16, 5386-97. 6 . MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20. 7 . Chaudhary, P.M. et al. (1997) Immunity 7, 821-30. 8 . Schneider, P. et al. (1997) FEBS Lett 416, 329-34. 9 . Shetty, S. et al. (2002) Apoptosis 7, 413-20. 10 . Sheridan, J.P. et al. (1997) Science 277, 818-21. 11 . Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70. 12 . MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20. 13 . Schneider, P. et al. (1997) FEBS Lett 416, 329-34. 14 . Pan, G. et al. (1998) FEBS Lett 424, 41-5. 15 . Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6. 16 . Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8. 17 . Walczak, H. et al. (1999) Nat Med 5, 157-63. 18 . Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62. |
Flow cytometric analysis of 786-0 cells using TRAIL (C92B9) Rabbit mAb (PE Conjugate) (blue) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red). |
