| 货号 | 32038S |
| 同种亚型 | Rabbit |
| 反应种属 | H M R Hm Mk B Dg |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | CDK9 (C12F7) Rabbit mAb (PE Conjugate) detects endogenous levels of total CDK9 protein, both 42 kDa and 55 kDa isoforms. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | P-TEFb is a general transcription factor that regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II). The P-TEFb complex is composed of a catalytic subunit, CDK9, and its regulatory cyclin partner, which can be cyclin T1, T2a, T2b or K (reviewed in 1,2). P-TEFb is recruited by the HIV Tat protein to allow transcriptional elongation, and subsequent replication of the viral genome. Inhibition of P-TEFb function therefore has potential for HIV therapy. CDK9 exists as two isoforms, an abundant 42 kDa isoform, and a less abundant 55 kDa isoform, which contains an amino-terminal extension (3). The two forms likely have distinct purposes based on differential expression during lymphocyte activation (4,5) and on their localization within the nucleus (5). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Rice, A.P. and Herrmann, C.H. (2003) Curr HIV Res 1, 395-404. 2 . De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7. 3 . Shore, S.M. et al. (2003) Gene 307, 175-82. 4 . Shore, S.M. et al. (2005) Gene 350, 51-8. 5 . Liu, H. and Herrmann, C.H. (2005) J Cell Physiol 203, 251-60. 6 . Chen, R. et al. (2004) J Biol Chem 279, 4153-60. 7 . Ammosova, T. et al. (2005) Retrovirology 2, 47. 8 . Fu, J. et al. (2007) Mol Cell Biol 27, 4641-51. 9 . De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7. |
Flow cytometric analysis of Jurkat cells using CDK9 (C12F7) Rabbit mAb (PE Conjugate) (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype Control (PE Conjugate) #5742 (red). |
