| 货号 | 51332S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB |
| 目标/特异性 | GAPDH (D4C6R) Mouse mAb (HRP Conjugate) recognizes endogenous levels of total GAPDH protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
| 参考文献 | 1 . Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95. 2 . Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38. 3 . Zheng, L. et al. (2003) Cell 114, 255-66. 4 . Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9. 5 . Wang, Q. et al. (2005) FASEB J. 19, 869-71. |
Western blot analysis of extracts from HeLa, COS-7, and 3T3 cell lines using GAPDH (D4C6R) Mouse mAb (HRP Conjugate). |
