| 货号 | 58289S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | F |
| 目标/特异性 | ELAVL1/HuR (D9W7E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total ELAVL1/HuR protein. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3 UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7). |
| 存放说明 | 4C |
| 参考文献 | 1 . Izquierdo, J.M. (2008) J Biol Chem 283, 19077-84. 2 . Fan, X.C. and Steitz, J.A. (1998) Proc Natl Acad Sci U S A 95, 15293-8. 3 . Peng, S.S. et al. (1998) EMBO J 17, 3461-70. 4 . Brennan, C.M. and Steitz, J.A. (2001) Cell Mol Life Sci 58, 266-77. 5 . Mazan-Mamczarz, K. et al. (2003) Proc Natl Acad Sci U S A 100, 8354-9. 6 . Lal, A. et al. (2005) EMBO J 24, 1852-62. 7 . Lebedeva, S. et al. (2011) Mol Cell 43, 340-52. |
Flow cytometric analysis of 293 cells (green) using ELAVL1/HuR (D9W7E) Rabbit mAb (PE Conjugate) (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (dashed line). |
