| 货号 | 54016S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | F |
| 目标/特异性 | EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of EGFR E746-A750del mutant protein. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur. |
| 存放说明 | 4C |
| 参考文献 | 1 . Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39. 2 . Pao W et al. (2004) Proc Natl Acad Sci U S A 101, 13306–11 3 . Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26. 4 . Kosaka, T. et al. (2004) Cancer Res 64, 8919-23. 5 . Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41. |
Flow cytometric analysis of KYSE-450 cells (blue) and HCC827 cells (green) using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (PE Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (dashed lines). |
