| 货号 | 53276S |
| 同种亚型 | Mouse IgG3 |
| 反应种属 | Human |
| 应用 | F |
| 目标/特异性 | CD133 (A8N6N) Mouse mAb (Flow Specific) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total CD133 protein. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8). |
| 存放说明 | 4C |
| 参考文献 | 1 . Yin, A.H. et al. (1997) Blood 90, 5002-12. 2 . Miraglia, S. et al. (1997) Blood 90, 5013-21. 3 . Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51. 4 . Monzani, E. et al. (2007) Eur J Cancer 43, 935-46. 5 . OBrien, C.A. et al. (2007) Nature 445, 106-10. 6 . Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5. 7 . Singh, S.K. et al. (2004) Nature 432, 396-401. 8 . Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34. |
Flow cytometric analysis of Ramos cells (blue) and WERI-Rb-1 cells (green) using CD133 (A8N6N) Mouse mAb (Flow Specific) (Alexa Fluor® 647 Conjugate) (solid lines) or a concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843 (dashed lines). |
